ABO Incompatible Heart Transplant Titer (anti A or anti B)

Indications Determination of ABO incompatible heart recipient’s isohemagglutinins (anti-A/ Anti-B) titer. The titer results assist in decisions pre transplant to minimize immunologic consequences of ABO incompatible heart transplant. 
Method Serial test tube dilution methodology
Test Information Isohemagglutinins Anti-A/Anti-B titers. Serial two-fold dilutions of serum are tested against A or B cells.Result is expressed as the reciprocal of the highest serum dilution at which (1+) agglutination is still present. 
Sample Requirements EDTA tube. Serum separator gel tube is not acceptable.
Requested Volume 1 full 7 ml EDTA sample
Minimum Volume or Pediatric volume Minimum volume: 1 full 7 ml EDTA sample
Pediatric: 1 full 3 ml EDTA sample; neonate: 2 full 0.5 ml EDTA microtainers
Shipping Information Ship at ambient temperature

Send samples to:
Bloodworks Northwest
Immunohematology Reference Laboratory
921 Terry Avenue
Seattle, WA 98104
Requisition Form RFT-Request for Testing Transfusion Services
Transaction Code 3115-00
CPT Codes 86886
Test Schedule Monday through Sunday
Turn around Time (analytic time) 24 hours if weekday; 48 hours if weekend
Report/ Results Fax number required to receive report