Direct Antiglobulin Test

Indications Detects the presence of red blood cell-bound IgG and/or complement (C3) by agglutination using an antiglobulin (Coombs) reagent. Positive results can occur in patients with autoimmune hemolytic anemia, drug-induced immune hemolysis (e.g., alpha-methyldopa, procainamide), hemolytic transfusion reactions, or hemolytic disease of the newborn.  Positive results can also occur due to non-specific IgG absorption (e.g., hypergamma-globulinemia), and occasionally in otherwise healthy individuals.  Elution testing is usually indicated after a positive result with IgG is detected.  
Method Standard test tube methodology, Hemagglutination
Test Information Extended evaluation may include elution and absorption studies.
Sample Requirements EDTA tube, separator gel tube is not acceptable.
Requested Volume 1 full 7 ml EDTA sample
Minimum Volume or Pediatric volume Minimum volume: 1 full 7 ml EDTA sample
Pediatric: 1 full 3 ml EDTA sample; neonate: 2 full 0.5 ml EDTA microtainers
Shipping Information Ship at ambient temperature

Send samples to:
Bloodworks Northwest
Immunohematology Reference Laboratory
921 Terry Avenue
Seattle, WA 98104
Requisition Form RFT-Request for Testing Transfusion Services
Transaction Code 3125-00
CPT Codes 86880
Test Schedule Monday through Sunday
Turn around Time (analytic time) 24 hours if weekday; 48 hours if weekend
Report/ Results Fax number required to receive report