Hemolysis Evaluation

Indications This battery can be ordered for clinical scenarios that include serological evaluation for possible clinical hemolysis such as a patient with a decrease in hematocrit that is unexplained by bleeding and supporting laboratory values (LDH, indirect bilirubin, haptoglobin) are suggestive of hemolysis or a patient who is being monitored due to the presence of a red cell autoantibody or a hemolytic anemia.
Method Sample will be evaluated by multiple methodologies
Test Information Initial testing consist of direct and indirect antiglobulin tests (DAT, IAT) if the previous testing was non-reactive. The investigation may include an elution with PEG-IAT, antibody identification if required, common phenotyping and in rare cases, red cell separation or RBC genomic testing if evidence of transfused cells is seen in the phenotyping.
Sample Requirements EDTA tube. Serum separator gel tube is not acceptable.
Requested Volume 2 full 7 ml EDTA sample
Minimum Volume or Pediatric volume Minimum volume: 1 full 7 ml EDTA sample
Pediatric: 1 full 3 ml EDTA sample; neonate: 2 full 0.5 ml EDTA microtainers
Shipping Information Ship at ambient temperature

Send samples to:
Bloodworks Northwest
Immunohematology Reference Laboratory
921 Terry Avenue
Seattle, WA 98104
Requisition Form RFT-Request for Testing Transfusion Services
Immunohematology Consultation Request
CPT Codes To be determined by tests performed
Test Schedule Monday through Sunday
Turn around Time (analytic time) TAT may vary depending on the complexity of the workup and patient status. Preliminary results available within 24 hours
Report/ Results Fax number required to receive report