Platelet Alloantibody Workup-Basic

Also Known as (Alias) Platelet Refractory Antibody
Method Flow Cytometry using beads coated with recombinant or soluble HLA antigens
Test Information Multiply transfused patients may become alloimmunized to foreign donors. Patients who have failed to respond to two consecutive random donor platelet transfusions may require HLA-selected platelet transfusions. Contact the Apheresis Donor Coordinator (425-453-5098) to schedule HLA-selected community donors. The Apheresis Scheduling Coordinator will ask you to send a blood sample from the patient to the Platelet Immunology Laboratory. HLA antibody tests will be used to detect alloantibodies.
Additional Test Information In antibody-negative patients, HLA matched platelet transfusions are not likely to improve transfusion responses as these patients are probably receiving drugs or have clinical factor(s) that prevent them from responding to platelets from any source. Such patients should be returned to random donor platelet transfusion therapy.

In antibody-positive patients who require HLA-selected donors must be HLA-typed. If the patient has not been HLA-typed, contact the Immunogenetics/HLA Laboratory (206-689-6580) for sample requirements.
Sample Requirements ACD (yellow top)
Clotted blood (red top)
Requested Volume 10cc ACD (yellow top) tube and 10cc clot (red top) tube
Shipping Information Send sample at ambient temperature and should be received in the lab 48 hours after collection

Send samples to:
Bloodworks Northwest
Attn:  Platelet Immunology Lab, Rm 619
921 Terry Avenue
Seattle, WA 98104

For additional information please see Ordering Information
Requisition Form RFT- Immunogenetics/HLA and Platelet Immunology Labs
Transaction Code 3630-00
CPT Codes 86832
Test Schedule Monday thru Friday
Turn around Time (analytic time) 1 business day
Report/Results Fax number required to receive report