Resolution of ABO discrepancy

Indications ABO testing discrepancies must be resolved prior to issuing of group specific RBC units. The range of testing required can vary greatly. ABO discrepancies can result from marrow / stem cell transplants which can be easily diagnosed and resolved when an accurate patient history is provided. They can be found in geriatric, malnourished or immunosuppressed patients.  ABO genetic variation can also be the cause of apparent discrepancies.
Method Multiple methodologies
Test Information Testing performed may range from repeating the ABO forward and reverse typing to increasing the ratio of reagent to sample, altering the incubation temperature, increasing incubation times, absorptions, elutions, inhibitions and determination of secretor status, family study in the case of a very rare subgroup or genomic testing. Communication with the facility caring for the patient will determine the necessity for complex testing and the urgency for provision of blood components. When necessary, Group O, Rh compatible units will be issued until the ABO type is confirmed.  RBC genotyping is also available.  
Sample Requirements EDTA tube. Serum separator gel tube is not acceptable.
Requested Volume 1 full 7ml EDTA sample
Minimum Volume or Pediatric volume Minimum volume: 1 full 7 ml EDTA sample
Pediatric: 1 full 3 ml EDTA sample; neonate: 2 full 0.5 ml EDTA microtainers
Shipping Information Ship at ambient temperature

Send samples to:
Bloodworks Northwest
Immunohematology Reference Laboratory
921 Terry Avenue
Seattle, WA 98104
Requisition Form RFT-Request for Testing Transfusion Services
Immunohematology Consultation Request
Test Schedule Monday through Sunday
Turn around Time (analytic time) TAT may vary depending on the complexity of the workup. 
Report/ Results Fax number required to receive report