Single Antigen Phenotype

Indications When determining the likelihood of hemolytic disease of the fetus and newborn in cases of maternal antibody, single antigen phenotyping can be ordered to find out if a father carries the antigen corresponding to a maternal antibody. Single antigen phenotyping can also be ordered in other scenarios where the red cell antigen to be typed is unknown.
Method Standard test tube methodology, Hemagglutination
Test Information Test dependent on the antigen present. Test is ordered for patient sample only.
Additional Test Information If the patient has been transfused in the last three months, antigen typing of patient red cells may yield unreliable results due to the presence of donor cells. A pre-transfusion should be used for antigen typing, if available.  RBC genotyping can provide this information in recently transfused recipients.
Sample Requirements EDTA tube. Serum separator gel tube is not acceptable.
Requested Volume 1 full 7 ml EDTA sample
Minimum Volume or Pediatric volume Minimum volume: 1 full 7 ml EDTA sample
Pediatric: 1 full 3 ml EDTA sample; neonate: 2 full 0.5 ml EDTA microtainers
Shipping Information Ship at ambient temperature

Send samples to:
Bloodworks Northwest
Immunohematology Reference Laboratory
921 Terry Avenue
Seattle, WA 98104
Requisition Form RFT-Request for Testing Transfusion Services
Transaction Code 3118-00
CPT Codes 86905
Test Schedule Monday through Sunday
Turn around Time (analytic time) 24 hours if weekday; 48 hours if weekend
Report/ Results Fax number required to receive report