|Indications||Determination of optimal temperature of reactivity of cold antibodies. *Special collection procedures are required.|
|Method||Standard test tube methodology, Hemagglutination|
The thermal amplitude test is performed to determine the reactivity of a cold autoantibody at varying temperatures: 4° C, 22° C, 30° C, and 37° C. Cold autoantibodies that are reactive at temperatures greater than 30° C have the potential to be clinically significant regardless of the antibody titer. When the IgM antibody reactions at greater than 30° C, it may be causing hemolysis (cold agglutinin syndrome, CAS). Cold antibodies that are reactive at temperatures less than 30° C are not considered to be clinically significant.
Please see sample information below for specific collection instructions
|Sample Requirements||EDTA tube. Serum separator gel tube is not acceptable.|
1 full 7 ml EDTA sample
|Minimum Volume or Pediatric volume||Minimum volume: 1 full 7 ml EDTA sample
Pediatric: 1 full 3 ml EDTA sample; neonate: 2 full 0.5 ml EDTA microtainers
Please call Immunohemtology Laboratory prior to drawing sample.
Sample drawn and maintained at 37°C. Once sample is separated, immediately transfer the plasma to a plastic tube labeled with patient's name and send to the lab.
|Shipping Information||Ship plasma at ambient temperature
Send samples to:
Immunohematology Reference Laboratory
921 Terry Avenue
Seattle, WA 98104
|Requisition Form||RFT-Request for Testing Transfusion Services|
|CPT Codes||86940 and 86941|
|Test Schedule||Monday through Sunday|
|Turn around Time (analytic time)||24 hours if weekday, 72 hours if weekend. This test is not performed on a STAT basis.|
|Report/ Results||Fax number required to receive report|