Thrombin Time (Abnormals reflex to 1:1 mix)

Profile or Test Panel
 Additional assays will be performed as indicated.
Abnormal result reflexes to the following tests as indicated.
Method Clottable
Test Information A low concentration of thrombin is added to plasma and the rate of reaction for the conversion of fibrinogen to fibrin is measured in seconds. The enzymatic cleavage of fibrin peptides is rate limiting because of the low concentration of thrombin. Prolongation of thrombin time can be caused by heparin, elevated levels of fibrin/fibrinogen degradation products (FDP), hereditary or acquired fibrinogen abnormalities (hypofibrinogenemia or dysfibrinogenemia), or the presence of fibrinolytic agents (e.g., tPA, urokinase).
Additional Test Information Normal Range:  18.0 - 24.0 sec
Sample Requirements Whole Blood:  3.2% Sodium Citrated (blue top)  OR
Frozen Plasma
Requested Volume Two 5 ml 3.2 sodium citrated (blue top) tubes as whole blood  OR
Frozen plasma in 4 X 1mL aliquots in plastic tubes 
Sample Information Whole blood is preferred for testing but if it is not possible to send whole blood tubes TO ARRIVE HERE WITHIN 3 HOURS of collection then send frozen plasma. To prepare plasma centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes.  Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze.  
Shipping Information Whole Blood:  Send at ambient temperature (18-25C) and must be received within the lab in less than or equal to 3 hours after collection.
Frozen Plasma:  send on dry ice 

Send samples to:

Bloodworks Northwest
Attn: Hemostasis Laboratory
921 Terry Ave
Seattle, WA 98104

For additional information, please see Ordering Information
Requisition Form RFT- Hemostasis Reference Laboratory
Transaction Code 3200-02
CPT Codes 85670
Turn around Time (analytic time) 5 Business Days
Report/ Results Fax number required to receive report