Platelet Autoantibody Test (ITP Workup)

Also Known as (Alias) Platelet Flow Cytometry
Method flow cytometry
Test Information Idiopathic Thrombocytopenic Purpura (ITP) is a diagnosis associated with elevated platelet associated IgG, IgM or both. Positive autoantibody test results are indicative of autoimmune destruction. Positive indirect antibody test results, using patient's plasma against normal random platelets, may indicate ITP depending upon the patient's transfusion or pregnancy history. In approximately 85% of patients with ITP the presence of an autoantibody can be detected
Additional Test Information Patients who require autoantibody testing must be platelet transfusion independent for approximately 5 days to ensure that the platelets being tested are the patient's own platelets and not circulating donor platelets.
Sample Requirements Citrate (blue top)
EDTA (lavender top)
Requested Volume 5cc Citrate (blue top)

EDTA (lavender top) for amount refer below
     Platelet Count
     Greater or equal to 50,000   Draw 20cc EDTA
     20,000 to 49,999                  Draw 30cc EDTA
     10,000 to 20,000                  Draw 40cc EDTA
     Less than 10,000                 DO NOT DRAW***

***We cannot recover sufficient platelets to perform a direct assay of antibody bound to  autologous platelets. Contact Lab Supervisor for testing options 
Sample Information Samples must be received Monday thru Friday morning.  Samples received after 10:00am on Friday or over the weekend will have to be re-drawn.  
Shipping Information Send whole blood at ambient temperature and should be received in the lab within 24 hours after collection

Send samples to:
Bloodworks Northwest
Attn:  Platelet Immunology Lab, Rm 619
921 Terry Avenue
Seattle, WA 98104

For additional information please see Ordering Information
Requisition Form RFT- Immunogenetics/HLA and Platelet Immunology Labs
Transaction Code 3640-00
CPT Codes 86022 x2, 86023 x2
Test Schedule Monday thru Friday morning
Turn around Time (analytic time) 1 business day
Report/Results Fax number required to receive report