Questions and Answers about Coronavirus

Prothrombin Time 1:1 Mix

Specialty Normal Range:  Contact Laboratory
Also Known as (Alias) Two 5 ml 3.2 sodium citrated (blue top) tubes as whole blood  OR
1 ml Frozen plasma into 4 plastic tubes
Method Clottable
Test Information The prothrombin time is performed using a 1:1 mix of patient plasma with normal plasma. Lack of prothrombin time correction with the  1:1 mix indicates the presence of a coagulation inhibitor.  If the prothrombin time is abnormal and etiology unclear, the testing will reflex to this test.
Additional Test Information Normal Range:  Contact Laboratory
Sample Requirements Whole Blood:  3.2% Sodium Citrated (blue top)  OR
Frozen Plasma
Requested Volume Two 5 ml 3.2 sodium citrated (blue top) tubes as whole blood  OR
Frozen plasma in 4 X 1mL aliquots in plastic tubes
Sample Information Whole blood is preferred for testing but if it is not possible to send whole blood tubes TO ARRIVE HERE within 3 hours of collection then send frozen plasma:  Centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes.  Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze.
Shipping Information Whole Blood:  Send at ambient temperature (18-25C) and must be received within the lab in less than or equal to 3 hours after collection.
Frozen Plasma:  send on dry ice

Send samples to:

Bloodworks Northwest
Attn: Hemostasis Laboratory
921 Terry Ave
Seattle, WA 98104

For additional information, please see Ordering Information

Requisition Form RFT- Hemostasis Reference Laboratory
Transaction Code 3200-04
CPT Codes 85610
Turn around Time (analytic time) 5 Business Days
Report/ Results Fax number required to receive report

Your gift of blood, time or money saves lives.