Factor II Activity

Profile or Test Panel
Extrinsic Factor (abnormal PT) Evaluation Profile
Method Clottable
Test Information A one-stage extrinsic system clotting assay is used to measure the circulating factor II (prothrombin) level. Clotting times of dilutions of patient plasma are compared to dilutions of a plasma pool from normal donors, when added to deficient plasma. Low values can be caused by hereditary or acquired factor II deficiency, factor II inhibitor, warfarin therapy, disseminated intravascular coagulation (DIC), vitamin K deficiency or liver disease.
Additional Test Information Normal ranges are included on testing report and can be made available upon request.
Sample Requirements Whole Blood: 3.2% sodium citrated (blue top) OR
frozen plasma.
Requested Volume Two 5 ml 3.2% sodium citrated (blue top) tubes as whole blood OR
frozen plasma in 4 X 1mL aliquots in plastic tubes
Minimum Volume or Pediatric Volume Frozen Plasma: at least 0.5ml in 2 plastic tubes.
Sample Information Whole blood is preferred for testing, but if it is not possible to send whole blood tubes TO ARRIVE HERE WITHIN 2 HOURS of collection, then send frozen plasma. To prepare plasma, centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes. Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze.
Shipping Information Whole Blood: Send at ambient temperature (18-25°C) and must be received within the lab in less than or equal to 2 hours after collection.

Frozen Plasma: Send on dry ice.

Send samples to:
Bloodworks Northwest
Attn: Hemostasis Laboratory
921 Terry Ave
Seattle, WA 98104

For additional information, please see Ordering Information

Requisition Form RFT- Hemostasis Reference Laboratory
Transaction Code 3210-10
CPT Codes 85210
Test Schedule Monday – Friday
Turn around Time (analytic time) 3 Business Day
Report/Results Fax number required to receive report

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