Bloodworks Research Institute

Flow Cytometry Core

Welcome to the Flow Cytometry Core at Bloodworks Northwest. Our mission is to empower researchers in advancing their studies of molecular characterization and functional analysis of cells, subcellular organelles, and small particles. Leveraging cutting-edge technology, including flow cytometry analyzers, cell sorters, and image flow cytometers, we are committed to facilitating breakthroughs in your research.

We offer a range of services to meet the diverse needs of both internal and external researchers.

Our services include:

• Quantitative analysis, cell sorting and imaging through the use of our advanced instruments.
• Consultation services with personalized consultation on protocol development and study design, tailored to enhance the success of your research.
• Hands-on training conducted by our experienced professionals, ensuring proficiency in utilizing all our instruments.

Instruments

1. Cytoflex LX is a flow cytometry analyzer with automated features
The Cytoflex LX has five lasers and a total of 18 channels. The lasers are 375, 405, 488, 561, and 638 nm. Samples can be run from an Eppendorf tube or a multi-well plate. Use of this machine is $75.00/hr for internal users and $180.00/hr for external users.

2. BD LSR II a flow cytometry analyzer
The BD LSR II has four lasers and a total of 12 channels. The lasers are blue, red, violet, and UV. Use of this machine is $35.00/hr for internal users and $84.00/hr for external users.

3. BD FACS Aria Cell Sorter
The BD FACS Aria is a cell sorter with four lasers and 17 total channels. The lasers are blue, red, yellow, and violet. Please contact us for services.

4. Amnis ImageStream X Mk II is an image flow cytometer
The Amnis Imagestream has four lasers and 12 channels. The lasers are 405, 488, 561, and 642 nm. The machine can capture cell images with 20X, 40X, or 60X magnification. Use of this machine is $100.00/hr for internal users and $240.00/hr for external users.