Donath-Landsteiner Test for PCH
|Indications||This test may be ordered in a patient suspected of having paroxysmal cold hemoglobinuria (PCH). This entity is more common in children, and may be characterized by episodes of hemolysis upon exposure to cold temperatures.
*Special collection procedures are required.
|Method||Standard test tube methodology, Hemagglutination|
|Test Information||As PCH is classically due to an IgG antibody, preliminary tests such as a direct antiglobulin test using polyspecific Coombs reagent may be performed prior to proceeding with the Donath-Landsteiner test; this is to help differentiate PCH from cold agglutinin syndrome (CAS), when an IgM antibody is causing hemolysis.
*Please see sample information below for specific collection instructions.
|Sample Requirements||Plain red top (clotted) tube. Serum separator gel tube is not acceptable.|
|Requested Volume||10 ml clotted sample
*Please see Sample information below
|Minimum Volume or Pediatric volume||10 ml clotted sample|
|Sample Information||Please call Immunohematology Laboratory prior to drawing sample
Sample drawn and maintained at 37°C. Once sample is clotted, separate serum from clot immediately and transfer plastic tube labeled with patient’s name and send to the lab.
|Shipping Information||Ship serum at ambient temperature
Send samples to:
|Requisition Form||Request for Testing-Immunohematology Reference Laboratory|
|CPT Codes||86940 and 86941|
|Test Schedule||Monday through Sunday|
|Turn around Time (analytic time)||24 hours if weekday, 72 hours if weekend. This test is not performed on a STAT basis.|
|Report/ Results||Fax number required to receive report|