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Platelet Immunology Laboratory

Sample Requirements

Tests Recommended Sample Age Minimum Sample Volume Comments
Platelet Alloantibody Workup (Basic or Advanced) 48 hours 10cc ACD (light yellow top)

10cc Clot (red top)

For Patients Receiving HLA-Matched Platelet Support. Always send whole blood, room temperature
Platelet Autoantibody Test (Direct and Indirect) 24 hours 5cc Citrate (blue top)

EDTA (lavender top) See below for amount:

Platelet Count Volume in EDTA
Greater or equal to 50,000/µl 20cc
20,000 to 49,999 30cc
10,000 to 19,999 40cc
Less than 10,000 *Do not draw
*If the platelet count is less than 10,000 we cannot recover sufficient platelets to perform the autoantibody test (Direct). Please contact the Lab Supervisor for your testing options.

Always send whole blood, room temperature.

 

Platelet Drug Antibody Test (Drug-related ITP) 24 hours See comments Please contact the Lab Supervisor for the testing requirements.

Always send whole blood, room temperature.

Neonatal Alloimmune Throm-
bocytopenia Evaluation (NATP)
*
24 hours Mother: 20cc EDTA (Lavender top); 10cc Citrate (blue top); 10cc Clot (Red top).

Father: 20cc EDTA (Lavender top)

Please contact the Lab Supervisor for
consultation and/or provide clinical history.
Always send whole blood, room temperature.
Post Transfusion Purpura Workup (PTP) 24 hours 10cc EDTA (Lavender top); 10cc Citrate (blue top); 10cc Clot (red top). Please contact the Lab Supervisor for the testing requirements.

Always send whole blood, room temperature.

Heparin Antibody Test (HIT) 24 hours 5cc Citrate
(blue top)**
**If sending over the weekend, spin and freeze plasma at -20°C then send frozen on dry ice.
Platelet Typing for Single Platelet Antigen 48 hours 10cc EDTA (lavender top) or 10cc ACD (light yellow top) Always send whole blood, room temperature.
ADAMTS-13
Protease
Activity
/ Inhibitor
Spin within 4 hours 5cc Citrate
(blue top)*
Spin and freeze plasma at -20oC then send frozen on dry ice.

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