Factor XIII Screen
| Profile or Test Panel |
Bleeding Diathesis with a Normal APTT/PT Profile
|
| Method | Clot Solubility |
| Test Information | Factor XIII forms covalent cross links between fibrin chains. In the absence of Factor XIII, the fibrin clot will be dissolved by 5 M urea which disrupts the hydrogen bonds. This assay will be abnormal only if the factor XIII level is <2-5%. Hereditary factor XIII deficiency, an autosomal recessive disorder, should be suspected in a patient with delayed post-surgical or traumatic bleeding, unexplained CNS hemorrhage. Women with severe FXIII deficiency have difficulty maintaining pregnancy. |
| Additional Test Information | Normal ranges are included on testing report and can be made available upon request. |
| Sample Requirements | Whole Blood: 3.2% sodium citrated (blue top) OR frozen plasma |
| Requested Volume | Two 5 ml 3.2% sodium citrated (blue top) tubes as whole blood OR frozen plasma in 4 X 1mL aliquots in plastic tubes |
| Minimum Volume or Pediatric Volume | Frozen Plasma: at least 0.5ml in 2 plastic tubes. |
| Sample Information | Whole blood is preferred for testing, but if it is not possible to send whole blood tubes TO ARRIVE HERE WITHIN 2 HOURS of collection, then send frozen plasma. To prepare plasma, centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes. Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze. |
| Shipping Information | Whole Blood: Send at ambient temperature (18-25°C) and must be received within the lab in less than or equal to 2 hours after collection.
Frozen Plasma: Send on dry ice Send samples to: For additional information, please see Ordering Information |
| Requisition Form | RFT- Hemostasis Reference Laboratory |
| Transaction Code | 3200-07 |
| CPT Codes | 85291 |
| Test Schedule | Run monthly |
| Turn around Time (analytic time) | Contact laboratory |
| Report/Results | Fax number required to receive report |
