|Profile or Test Panel|
|Test Information||A one-stage extrinsic system clotting assay is used to measure the factor V level. Clotting times of dilutions of patient plasma are compared to dilutions of a plasma pool from normal donors, when added to deficient plasma. Levels are reduced by hereditary or acquired factor V deficiency, factor V inhibitor (including those seen after use of topical bovine thrombin), liver disease, or disseminated intravascular coagulation (DIC). The factor V (Leiden) mutation associated with activated protein C resistance does not affect factor V activity and is tested by separate kinetic or DNA testing (described later).|
|Additional Test Information||Normal Range 78-131% effective 11/1/11|
|Sample Requirements||Whole Blood: 3.2% Sodium Citrated (blue top) OR
|Requested Volume||Two 5 ml 3.2 sodium citrated (blue top) tubes as whole blood OR
Frozen plasma in 4 X 1mL aliquots in plastic tubes
|Minimum Volume or Pediatric volume||Frozen Plasma: at least 0.5ml in 2 plastic tubes.|
|Sample Information||Whole blood is preferred for testing but if it is not possible to send whole blood tubes TO ARRIVE HERE WITHIN 3 HOURS of collection then send frozen plasma. To prepare plasma centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes. Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze.|
|Shipping Information||Whole Blood: Send at ambient temperature (18-25C) and must be received within the lab in less than or equal to 3 hours after collection.
Frozen Plasma: send on dry iceSend samples to:
For additional information, please see Ordering Information
|Requisition Form||RFT- Hemostasis Reference Laboratory|
|Test Schedule||Monday – Friday|
|Turn around Time (analytic time)||1 Business Day|
|Report/ Results||Fax number required to receive report|
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