Questions and Answers about Coronavirus

Factor V Activity

Profile or Test Panel
Extrinsic Factor (abnormal PT) Evaluation Profile
Method Clottable
Test Information A one-stage extrinsic system clotting assay is used to measure the factor V level. Clotting times of dilutions of patient plasma are compared to dilutions of a plasma pool from normal donors, when added to deficient plasma. Levels are reduced by hereditary or acquired factor V deficiency, factor V inhibitor (including those seen after use of topical bovine thrombin), liver disease, or disseminated intravascular coagulation (DIC). The factor V (Leiden) mutation associated with activated protein C resistance does not affect factor V activity and is tested by separate kinetic or DNA testing (described later).
Additional Test Information Normal Range 78-131%  effective 11/1/11
Sample Requirements Whole Blood:  3.2% Sodium Citrated (blue top)  OR
Frozen Plasma
Requested Volume Two 5 ml 3.2 sodium citrated (blue top) tubes as whole blood  OR
Frozen plasma in 4 X 1mL aliquots in plastic tubes
Minimum Volume or Pediatric volume Frozen Plasma:  at least 0.5ml in 2 plastic tubes.
Sample Information Whole blood is preferred for testing but if it is not possible to send whole blood tubes TO ARRIVE HERE WITHIN 3 HOURS of collection then send frozen plasma.  To prepare plasma centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes.  Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze.
Shipping Information Whole Blood:  Send at ambient temperature (18-25C) and must be received within the lab in less than or equal to 3 hours after collection.
Frozen Plasma:  send on dry iceSend samples to:

Bloodworks Northwest
Attn: Hemostasis Laboratory
921 Terry Ave
Seattle, WA 98104

For additional information, please see Ordering Information

Requisition Form RFT- Hemostasis Reference Laboratory
Transaction Code 3210-11
CPT Codes 85220
Test Schedule Monday – Friday
Turn around Time (analytic time) 1 Business Day
Report/ Results Fax number required to receive report

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