Factor VII Activity
| Profile or Test Panel |
Extrinsic Factor (abnormal PT) Evaluation Profile
|
| Method | Clottable |
| Test Information | A one-stage extrinsic system clotting assay is used to measure circulating factor VII level. Clotting times of dilutions of patient plasma are compared to dilutions of a plasma pool from normal donors, when added to deficient plasma. Levels are reduced by hereditary or acquired factor VII deficiency, vitamin K deficiency, liver disease, warfarin therapy, or disseminated intravascular coagulation (DIC). |
| Additional Test Information | Normal ranges are included on testing report and can be made available upon request. |
| Sample Requirements | Whole Blood: 3.2% sodium citrated (blue top) OR frozen plasma |
| Requested Volume | Two 5 ml 3.2% sodium citrated (blue top) tubes as whole blood OR frozen plasma in 4 X 1mL aliquots in plastic tubes |
| Minimum Volume or Pediatric Volume | Frozen Plasma: at least 0.5ml in 2 plastic tubes. |
| Sample Information | Whole blood is preferred for testing, but if it is not possible to send whole blood tubes TO ARRIVE HERE WITHIN 2 HOURS of collection, then send frozen plasma. To prepare plasma centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes. Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze. |
| Shipping Information | Whole Blood: Send at ambient temperature (18-25°C) and must be received within the lab in less than or equal to 2 hours after collection.
Frozen Plasma: Send on dry ice Send samples to: For additional information, please see Ordering Information |
| Requisition Form | RFT- Hemostasis Reference Laboratory |
| Transaction Code | 3210-12 |
| CPT Codes | 85230 |
| Test Schedule | Monday – Friday |
| Turn around Time (analytic time) | 3 Business Day |
| Report/Results | Fax number required to receive report |
