HIV 1/2 Confirmatory

Indications Confirmatory testing of samples found to be repeatedly reactive by an anti-HIV-1/HIV-2 assay.
Method Bio-Rad GS HIV-1 Western Blot
Test Information Assay is a qualitative assay for the detection of antibodies to Human Immunodeficiency Virus Type 1 in human serum or plasma specimens.
Sample Requirements Serum or Plasma (EDTA, ACD, CPD, and CPDA)
Requested Volume 6 mL
Minimum Volume or Pediatric volume 1 mL
Shipping Information Samples are acceptable at 2-8°C for up to 7 days; samples may be frozen at -20 °C
Requisition Form Donor Testing RFT
Transplant Infectious Disease Testing Request (Contact the Donor Testing Laboratory @425-656-7907)
Transaction Code 3075-04
CPT Codes 86689
Test Schedule Referral Laboratory
Turn around Time (analytic time) Varied, dependent upon referral laboratory testing schedule.  Samples are shipped weekly.
Report/ Results Positive, Indeterminate, Negative or Unreadable.
Also Known as (Alias) anti-HIV-2
Indications Confirmatory testing of samples found to be repeatedly reactive by an anti-HIV-1/HIV-2 assay.
Method BioRad GS HIV-2 EIA
Test Information Assay is an enzyme immunoassay for the detection of antibodies to Human Immunodeficiency Virus Type 2 in human serum or plasma.
Additional Test Information This test is performed when the anti-HIV -1/-2 is positive and the Fluorognost HIV-1 IFA is negative.
Sample Requirements Serum or Plasma
Requested Volume 6 mL
Minimum Volume or Pediatric volume 1 mL
Shipping Information Samples are acceptable at 2-8°C for 7 days; samples may be frozen at -20 °C or lower.
Requisition Form Donor Testing RFT
Transplant Infectious Disease Testing Request (Contact the Donor Testing Laboratory @425-656-7907)
Transaction Code 3075-04
CPT Codes 86702
Test Schedule Every 2-3 weeks
Turn around Time (analytic time) Varied, depending on batch size
Report/ Results Reactive or Nonreactive
Also Known as (Alias) HIV Supplemental Testing
Indications Confirmatory testing of samples found to be repeatedly reactive by an anti-HIV-2 assay.
Method BioRad Geenius  HIV 1/2 Supplemental Assay
Test Information A Qualitative Assay for the Confirmation and Differentiation of individual Antibodies to HIV-1 and HIV-2 in whole blood, serum, or plasma Speciments.
Additional Test Information This is a referral test that is used only when the anti-HIV-1/-HIV-2 is positive, the Fluorognost HIV-1 IFA is negative, and the Bio-Rad anti-HIV-2 test is positive.
Sample Requirements Serum or Plasma (EDTA, heparin, or NaCitrate)
Requested Volume 6 mL
Minimum Volume or Pediatric volume 1 mL
Shipping Information Samples are acceptable at 2-8°C for 7 days; samples may be frozen at -20 °C.
Requisition Form Donor Testing RFT
Transplant Infectious Disease Testing Request (Contact the Donor Testing Laboratory @425-656-7907)
Transaction Code 3075-04
CPT Codes 86702
Test Schedule Referral Laboratory
Turn around Time (analytic time) Varied, dependent on Referral Laboratory schedule
Report/ Results HIV Negative, HIV-1 Indeterminate, HIV-2 Indeterminate, HIV Indeterminate, HIV-1 Positive, HIV-2 Positive, HIV-2 Positive with HIV-1 cross-reactivity, HIV Positive undifferentiated.

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