Prothrombin Time 1:1 Mix
| Indications | ORDERED BY LAB AS A REFLEXIVE TEST ONLY |
| Method | Clottable |
| Test Information | The prothrombin time is performed using a 1:1 mix of patient plasma with normal plasma. Lack of prothrombin time correction with the 1:1 mix indicates the presence of a coagulation inhibitor. If the prothrombin time is abnormal and etiology unclear, the testing will reflex to this test. |
| Additional Test Information | Normal ranges are included on testing report and can be made available upon request. |
| Sample Requirements | Whole Blood: 3.2% sodium citrated (blue top) OR frozen plasma. |
| Requested Volume | Two 5 ml 3.2% sodium citrated (blue top) tubes as whole blood OR frozen plasma in 4 X 1mL aliquots in plastic tubes. |
| Sample Information | Whole blood is preferred for testing, but if it is not possible to send whole blood tubes TO ARRIVE HERE within 2 hours of collection, then send frozen plasma. To prepare plasma, centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes. Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze. |
| Shipping Information | Whole Blood: Send at ambient temperature (18-25°C) and must be received within the lab in less than or equal to 2 hours after collection.
Frozen Plasma: Send on dry ice Send samples to: For additional information, please see Ordering Information. |
| Requisition Form | RFT- Hemostasis Reference Laboratory |
| Transaction Code | 3200-04 |
| CPT Codes | 85611 |
| Test Schedule | Monday-Friday |
| Turn around Time (analytic time) | 3 Business Days |
| Report/Results | Fax number required to receive report |
