Rh Genotyping- Rh C,D,E Systems

Also Known as (Alias) Rh Genotyping
Indications Red Cell (HEA) Genomics
Method polymerase chain reaction (PCR) based
Test Information Fetal samples can be genotyped for compatibility with mothers with RH isoimmunization.
Additional Test Information Fetal Cell Genotyping:
Requires cultured amniocytes in two T-25 flasks grown to confluency (amniotic fluid not acceptable).  We do not provide laboratory services to culture the amniocytes.

Mother:
10 cc EDTA (lavender top) tube for maternal cell contamination.

*Note:  For shipping, the cultured cells should have media added to the flasks and sealed to avoid leakage

Sample Requirements EDTA (lavender top)
Requested Volume 10cc EDTA (lavender top) tube(s)
Sample Information 5cc EDTA
Shipping Information 5-10cc EDTA (lavender top) tube

If analysis of cultured amniocyte is requested, EDTA must also be drawn on mother of fetus. Two T-25 flasks of cells cultured to confluency will be accepted. Bloodworks Northwest does not have facilities to culture amniocytes.  Bloodworks Northwest does not have facilities to culture amniocytes.

Requisition Form RFT: Genomics Testing Laboratory (for Red Cell testing)
Transaction Code 3117-02
CPT Codes 81401 x5
Turn around Time (analytic time) 3 business days
Minimum Volume or Pediatric volume Minimum Vol: 5ml

Pediatric Minimum Vol: 2ml or 4 EDTA (lavender) microtainers, each containing 0.5ml

*Note: For very difficult draws, contact the Genomics Testing Lab at Bloodworks NW at 206-689-6269 for approval of microtainers

Report/Results Fax number required to receive report
Limitation of test Molecular testing to predict the red blood cell phenotype does not always reflect the phenotypic expression of the blood group antigen. This may occur in cases where the individual has genetic variation that silences or alters the blood group antigen expression that is not included in the target region of the test, and also may occur with RhD zygosity testing. Test results must be interpreted with knowledge of how known polymorphisms affect blood group antigen expression. The performance characteristics have been established by Bloodworks Northwest. The results are not intended as the sole means for clinical diagnosis for donor or patient management decisions.

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