The Flow Cytometry Laboratory (Flow Lab) provides cell sorting and analysis services for in-house and external investigators. The Flow Lab staff operates the sorter and assists investigators using the analyzers. The staff also provides consultation regarding cytometry experimental design, sample preparation and data analysis. In addition, the staff welcomes the opportunity for external research project contracts.
The Flow Lab is equipped with FACSAria cell sorter, LSR II cytometer and Amnis ImageStream Mk II imaging cytometry system. The lab is directed by cell analysis specialist Dr. Xiaoping Wu. Prior to joining BloodworksNW Research Institute, Dr. Wu had extensive research training in Immunology and Molecular Biology.
The Flow Lab has been actively involved in ongoing and previous projects addressing bleeding disorder, transfusion complications, coagulopathy in traumatic brain injury, sickle cell disease, and thrombosis, megakaryocytopoiesis, platelet generation, autoimmunity and therapeutic immune modulation for inhibitory antibodies against factor VIII.
The Lab is located in Room 1136 in the Research Institute of the BloodworksNW at 1551 Eastlake Ave E, Seattle, WA 98102.
The staff requires information about the use of infectious agents, sample sources and preparation procedures before accepting samples for processing in the Flow Lab.
Service can be reserved via phone (206) 568-2238, lab (206) 568-2162 or email firstname.lastname@example.org.
Reserved service can be canceled no later than 3 hours in advance of the scheduled time. If not canceled, half of the fee for the reserved time will be charged.
|Cytometers||Lasers||Commonly Detected Dyes|
|FACSAria cell sorter||488nm, 633nm, 561nm and 405nm||FITC, PE, PerCP-Cy5.5, APC, Alexa 700, APC-Cy7, PE-TexRed/mcherry, PE-Cy5, PE-Cy5.5, PE-Cy7, Pacific Blue, AmCyn, Qdot605 and Qdot655|
|LSR II analyzer||488nm, 633nm, 405nm and 355nm||FITC, PE, PerCP/PE-Cy5/PerCP-Cy5.5, PE-Cy7, APC, Alexa Fluor700, APC-Cy7, Pacific Blue, AmCyn, BV605, Indo-Violet and Indo-Blue.|
|Amnis ImageStream Mk II||488nm, 561nm, 642nm and 405nm||FITC, PE, PE-TexRed/m-cherry, PerCP-Cy5.5, PE-Cy7, Pacific Blue/DAPI, AmCyn, efluor625/BV605, APC and APC-Cy7|
Dr. Xiaoping Wu, cell analysis specialist of the Flow Cytometry Core, is conducting experiments on our flow cytometers.
Funded by M. J. Murdock Charitable Trust, Dorothy F. Thorne Foundation, Seattle Foundation Medical Funds and BloodworksNW Faces For Life events, our high-performance FACSAria cell sorter, the LSR II analyzer and two other cytometers, FACSCalibur and FACSVantage, are essential tools for BloodworksNW researchers in thrombotic and bleeding disorders, and transfusion medicine.
Amnis ImageStream Mk II
BD FACSAria cell sorter
BD LSR II cytometer
1551 Eastlake Avenue E, Suite 100
Seattle, WA 98102
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