Chung Laboratory

Dominic Chung, Ph.D.

The Chung laboratory studies plasma proteins involved in blood coagulation; abnormal function in and regulation of these proteins cause bleeding and thrombotic diseases. An improved understanding of how these proteins function may lead to improved ways of preventing and treating these disorders.

Focus Areas of Study

  • Role of apolipoproteins in thrombosis of small vessels
  • Biomarkers of vessel activation and thrombotic diseases
  • Stabilization of plasma proteins

Recent Findings

  • High density lipoprotein particles (HDL) can attenuate thrombosis of small vessels by preventing the formation of hyperadhesive von Willebrand factor fibers in the microcirculation.
  • High circulating levels of syndecan-1 are present in patients with cerebral malaria; the high levels correlate with (i) the severity of vessel damage, (ii) low platelet counts, and (iii) low levels of apolipoprotein associated with HDL.

Dr Chung’s Background

  • Joined the Bloodworks Northwest Research Institute as a full member in Dec 2012
  • Professor of Biochemistry at the University of Washington.

Representative Publications

  1. Fujikawa K, Suzuki H, McMullen B, Chung D. Purification of human von Willebrand factor-cleaving protease and its identification as a new member of the metalloproteinase family. Blood. 2001; 98(6):1662-6.
  2. Zheng X, Chung D, Takayama TK, Majerus EM, Sadler JE, Fujikawa K. Structure of von Willebrand factor-cleaving protease (ADAMTS13), a metalloprotease involved in thrombotic thrombocytopenic purpura. J Biol Chem. 2001; 276(44):41059-63.
  3. Chung DW, Fujikawa K. Processing of von Willebrand factor by ADAMTS-13. Biochemistry. 2002; 41(37):11065-70.
  4. Wu JJ, Fujikawa K, Lian EC, McMullen BA, Kulman JD, Chung DW. A rapid enzyme-linked assay for ADAMTS-13. J Thromb Haemost. 2006; 4(1):129-36.
  5. Wu JJ, Fujikawa K, McMullen BA, Chung DW. Characterization of a core binding site for ADAMTS-13 in the A2 domain of von Willebrand factor. Proc Natl Acad Sci U S A. 2006;103(49):18470-4.
  6. Chen J, Fu X, Wang Y, Ling M, McMullen B, Kulman J, Chung DW, López JA. Oxidative modification of von Willebrand factor by neutrophil oxidants inhibits its cleavage by ADAMTS13. Blood. 2010;115(3):706-12.
  7. Fu X, Chen J, Gallagher R, Zheng Y, Chung DW, López JA. Shear stress-induced unfolding of VWF accelerates oxidation of key methionine residues in the A1A2A3 region. Blood. 2011; 118(19): 5283-91.
  8. Chen J, Ling M, Fu X, López JA, Chung DW. Simultaneous exposure of sites in von Willebrand factor for glycoprotein Ib binding and ADAMTS13 cleavage: studies with ristocetin. Arterioscler Thromb Vasc Biol. 2012; 32(11):2625-30.
  9. Interlandi G, Ling M, Tu AY, Chung DW, Thomas WE. Structural basis of type 2A von Willebrand disease investigated by molecular dynamics simulations and experiments. PLoS One. 2012;7(10):e45207. doi: 10.1371/journal.pone.0045207. Epub 2012 Oct 23.
  10. Weigandt KM, White N, Chung D, Ellingson E, Wang Y, Fu X, Pozzo DC. Fibrin clot structure and mechanics associated with specific oxidation of methionine residues in fibrinogen. Biophys J. 2012; 103(11): 2399-2407.
  11. Chen J, Chung DW, Le J, Ling M, Konkle BA, López JA. Normal Cleavage of von Willebrand Factor by ADAMTS13 in the absence of Factor VIII in Patients with Severe Hemophilia A. J Thromb Haemost. 2013 May 18. doi: 10.1111/jth.12299. [Epub ahead of print]

Chung Laboratory Members

Minhua Ling, Ph.D.
Research Scientist
Email: minhual@bloodworksnw.org
Phone: (206) 568-2166
FAX: (206) 587-6056

Dominic Chung, Ph.D.
Full Member, Bloodworks Northwest Research Institute

Professor of Biochemistry, University of Washington School of Medicine

Bloodworks Northwest
Research Institute
1551 Eastlake Avenue E, Suite 100
Seattle, WA 98102

Email: Chung@bloodworksnw.org
UW Mailstop 359190
Phone: (206) 568-2247
FAX: (206) 587-6056

Rachel A. Sessum
Sr. Administrative Coordinator
Email: rachels@bloodworksnw.org
Phone: (206) 568-2246
FAX: (206) 587-6056

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