Antibody Identification
| Indications | Red blood cell antibody specificity is determined by reacting patient serum with extended panel of reagent red blood cells of known RBC phenotype. Clinically significant alloantibodies (e.g., Rh, Kell, Duffy, Kidd) may be present in patients with hemolytic transfusion reactions or hemolytic disease of the newborn. The clinical significance of some of the more commonly encountered red cell alloantibodies are summarized in the Clinically Significant Red Cell Antibodies table. Autoantibodies usually react with all panel cells (i.e., “panagglutinins”), and are present in the sera of ~80% of patients with warm (IgG) autoimmune hemolytic anemia. Red cell alloantibodies may require several techniques for final identification. The presence of an autoantibody may require a series of absorptions and elutions to determine the presence of underlying alloantibodies. |
| Method | Red cell antibody identification by multiple methodologies |
| Test Information | This test panel may include other tests such as DAT, elution, adsorptions and RBC genotyping depending on the complexity of the workup. An additional fee may be added. |
| Sample Requirements | EDTA or plain red top (clotted) tube. Serum separator gel tube is not acceptable. |
| Requested Volume | 20 ml EDTA or 20ml clotted sample |
| Minimum Volume or Pediatric volume | Minimum volume: 20 ml EDTA or 10 ml clotted sample Pediatric: 1 full 3 ml EDTA sample; neonate: 2 full 0.5 ml EDTA microtainers |
| Shipping Information | Ship at ambient temperature
Send samples to: |
| Requisition Form | Request for Testing-Immunohematology Reference Laboratory Immunohematology Consultation Request |
| CPT Codes | To be determined by tests performed |
| Test Schedule | Monday through Sunday |
| Turn around Time (analytic time) | STAT: 8 hours after receipt in laboratory ASAP: 24 hours if weekday Routine: 2-5 days The times frames stated are guidelines and TAT will vary depending on the complexity of the workup and patient status. Preliminary results available within 24 hours. |
| Report/ Results | Fax number required to receive report |
