Antibody Titer (other than anti A or anti B)

Also Known as (Alias) Red cell antibody titration
Indications Determination of the titer of clinically significant IgG (non-ABO) antibodies present in the patient’s plasma. Antibody Titration is also performed to confirm the presence HTLA antibodies.
Method Serial test tube dilution methodology
Test Information This test is most often performed on the plasma of pregnant women which contains an antibody(ies) implicated in causing Hemolytic Disease of the fetus and newborn (HDFN).Serial, two-fold dilutions of serum are tested against red cells carrying the antigen of interest (e.g., D, K) using the indirect antiglobulin method. Result is expressed as the reciprocal of the highest serum dilution which still shows (1+) agglutination. This test is used to monitor serum alloantibody levels in previously sensitized pregnant women for prenatal management of hemolytic disease of the newborn (HDN).Testing may be repeated at intervals throughout the pregnancy to monitor for a significant increase in titer.  In women with Rh alloimmunization, anti-D levels greater or equal to 16 are generally considered an indication for invasive fetal testing.
Additional Test Information For prenatal sample, antibody identification will be performed prior to titration studies.  Titration not indicated when antibody(ies) reacting weakly in saline tube test and will be reported as “too weak to titer”
Sample Requirements EDTA tube. Serum separator gel tube is not acceptable.
Requested Volume 1 full 7 ml EDTA
Minimum Volume or Pediatric volume Minimum volume: 1 full 7 ml EDTA sample
Shipping Information Ship at ambient temperature

Send samples to:
Bloodworks Northwest
Immunohematology Reference Laboratory
921 Terry Avenue
Seattle, WA 98104

Requisition Form Request for Testing-Immunohematology Reference Laboratory
Transaction Code 3115-00
CPT Codes 86886
Test Schedule Monday through Sunday
Turn around Time (analytic time) 24 hours if weekday; 48 hours if weekend
Report/ Results Fax number required to receive report

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