Platelet Alloantibody Workup-Basic
|Also Known as (Alias)||Platelet Refractory Antibody|
|Method||Flow Cytometry using beads coated with recombinant or soluble HLA antigens|
|Test Information||Multiply transfused patients may become alloimmunized to foreign donors. Patients who have failed to respond to two consecutive random donor platelet transfusions may require HLA-selected platelet transfusions. Contact the Apheresis Donor Coordinator (425-453-5098) to schedule HLA-selected community donors. The Apheresis Scheduling Coordinator will ask you to send a blood sample from the patient to the Platelet Immunology Laboratory. HLA antibody tests will be used to detect alloantibodies.|
|Additional Test Information||In antibody-negative patients, HLA matched platelet transfusions are not likely to improve transfusion responses as these patients are probably receiving drugs or have clinical factor(s) that prevent them from responding to platelets from any source. Such patients should be returned to random donor platelet transfusion therapy.
In antibody-positive patients who require HLA-selected donors must be HLA-typed. If the patient has not been HLA-typed, contact the Immunogenetics/HLA Laboratory (206-689-6580) for sample requirements.
|Sample Requirements||ACD (yellow top)
Clotted blood (red top)
|Requested Volume||10cc ACD (yellow top) tube and 10cc clot (red top) tube|
|Shipping Information||Send sample at ambient temperature and should be received in the lab 48 hours after collection
Send samples to:
For additional information please see Ordering Information
|Requisition Form||RFT- Platelet Immunology Laboratory|
|Test Schedule||Monday thru Friday|
|Turn around Time (analytic time)||1 business day|
|Report/Results||Fax number required to receive report|