Antibody Identification

Indications Red blood cell antibody specificity is determined by reacting patient serum with extended panel of reagent red blood cells of known RBC phenotype. Clinically significant alloantibodies (e.g., Rh, Kell, Duffy, Kidd) may be present in patients with hemolytic transfusion reactions or hemolytic disease of the newborn. The clinical significance of some of the more commonly encountered red cell alloantibodies are summarized in the Clinically Significant Red Cell Antibodies table. Autoantibodies usually react with all panel cells (i.e., “panagglutinins”), and are present in the sera of ~80% of patients with warm (IgG) autoimmune hemolytic anemia. Red cell alloantibodies may require several techniques for final identification.  The presence of an autoantibody may require a series of absorptions and elutions to determine the presence of underlying alloantibodies.
Method Red cell antibody identification by multiple methodologies
Test Information This test panel may include other tests such as DAT, elution, adsorptions and RBC genotyping depending on the complexity of the workup. An additional fee may be added.
Sample Requirements EDTA or plain red top (clotted) tube. Serum separator gel tube is not acceptable.
Requested Volume 20 ml EDTA or 20ml clotted sample
Minimum Volume or Pediatric volume Minimum volume: 20 ml EDTA or 10 ml clotted sample
Pediatric: 1 full 3 ml EDTA sample; neonate: 2 full 0.5 ml EDTA microtainers
Shipping Information Ship at ambient temperature

Send samples to:
Bloodworks Northwest
Immunohematology Reference Laboratory
921 Terry Avenue
Seattle, WA 98104

Requisition Form Request for Testing-Immunohematology Reference Laboratory
Immunohematology Consultation Request
CPT Codes To be determined by tests performed
Test Schedule Monday through Sunday
Turn around Time (analytic time) STAT: 8 hours after receipt in laboratory
ASAP: 24 hours if weekday
Routine: 2-5 days
The times frames stated are guidelines and TAT will vary depending on the complexity of the workup and patient status. Preliminary results available within 24 hours.
Report/ Results Fax number required to receive report

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