Reptilase Time

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Fibrinogen Evaluation Profile
Indications Reflexive test may be used with an abnormal Thrombin time that does not correct on 1:1 mix
Method Clottable
Test Information Reptilase (a snake venom) is used to measure the rate of conversion of fibrinogen to fibrin. The Reptilase time is prolonged by hereditary or acquired fibrinogen abnormalities, elevated levels of fibrin/fibrinogen degradation products (FDP), and exogenous fibrinolytic agents, but is normal in the presence of heparin.
Additional Test Information Normal ranges are included on testing report and can be made available upon request.
Sample Requirements Whole Blood: 3.2% sodium citrated (blue top) OR
frozen plasma.
Requested Volume Two 5 ml 3.2% sodium citrated (blue top) tubes as whole blood OR
frozen plasma in 4 X 1mL aliquots in plastic tubes.
Sample Information Whole blood is preferred for testing, but if it is not possible to send whole blood tubes TO ARRIVE HERE WITHIN 2 HOURS of collection, then send frozen plasma. To prepare plasma, centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes. Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze.
Shipping Information Whole Blood: Send at ambient temperature (18-25°C) and must be received within the lab in less than or equal to 2 hours after collection.

Frozen Plasma: Send on dry ice

Send samples to:
Bloodworks Northwest
Attn: Hemostasis Laboratory
921 Terry Ave
Seattle, WA 98104

For additional information, please see Ordering Information

Requisition Form RFT- Hemostasis Reference Laboratory
Transaction Code 3200-11
CPT Codes 85635
Test Schedule Monday-Friday
Turn around Time (analytic time) 10 Business Days

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