Resolution of ABO discrepancy
| Indications | ABO testing discrepancies must be resolved prior to issuing of group specific RBC units. The range of testing required can vary greatly. ABO discrepancies can result from marrow / stem cell transplants which can be easily diagnosed and resolved when an accurate patient history is provided. They can be found in geriatric, malnourished or immunosuppressed patients. ABO genetic variation can also be the cause of apparent discrepancies. |
| Method | Multiple methodologies |
| Test Information | Testing performed may range from repeating the ABO forward and reverse typing to increasing the ratio of reagent to sample, altering the incubation temperature, increasing incubation times, absorptions, elutions, inhibitions and determination of secretor status, family study in the case of a very rare subgroup or genomic testing. Communication with the facility caring for the patient will determine the necessity for complex testing and the urgency for provision of blood components. When necessary, Group O, Rh compatible units will be issued until the ABO type is confirmed. RBC genotyping is also available. |
| Sample Requirements | EDTA tube. Serum separator gel tube is not acceptable. |
| Requested Volume | 1 full 7ml EDTA sample |
| Minimum Volume or Pediatric volume | Minimum volume: 1 full 7 ml EDTA sample Pediatric: 1 full 3 ml EDTA sample; neonate: 2 full 0.5 ml EDTA microtainers |
| Shipping Information | Ship at ambient temperature
Send samples to: |
| Requisition Form | Request for Testing-Immunohematology Reference Laboratory Immunohematology Consultation Request |
| Test Schedule | Monday through Sunday |
| Turn around Time (analytic time) | TAT may vary depending on the complexity of the workup. |
| Report/ Results | Fax number required to receive report |
