Thrombin Time (Abnormals reflex to 1:1 mix)
|Profile or Test Panel
Abnormal PT or aPTT Reflexive Evaluation Profile
Fibrinogen Evaluation Profile
Fibrinolysis Evaluation Profile
Lupus Anticoagulant Profile
|A low concentration of thrombin is added to plasma and the rate of reaction for the conversion of fibrinogen to fibrin is measured in seconds. The enzymatic cleavage of fibrin peptides is rate limiting because of the low concentration of thrombin. Prolongation of thrombin time can be caused by heparin, elevated levels of fibrin/fibrinogen degradation products (FDP), hereditary or acquired fibrinogen abnormalities (hypofibrinogenemia or dysfibrinogenemia), or the presence of fibrinolytic agents (e.g., tPA, urokinase).
|Additional Test Information
|Normal ranges are included on testing report and can be made available upon request.
|Whole Blood: 3.2% sodium citrated (blue top) OR
|Two 5 ml 3.2% sodium citrated (blue top) tubes as whole blood OR
frozen plasma in 4 X 1mL aliquots in plastic tubes.
|Whole blood is preferred for testing, but if it is not possible to send whole blood tubes TO ARRIVE HERE WITHIN 2 HOURS of collection, then send frozen plasma. To prepare plasma, centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes. Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze.
|Whole Blood: Send at ambient temperature (18-25°C) and must be received within the lab in less than or equal to 2 hours after collection.
Frozen Plasma: Send on dry ice
Send samples to:
For additional information, please see Ordering Information
|RFT- Hemostasis Reference Laboratory
|Turn around Time (analytic time)
|3 Business Days
|Fax number required to receive report