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Factor IX Activity

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Method Clottable
Test Information A one-stage intrinsic system clotting assay is used to measure circulating factor IX levels. Clotting times of dilutions of patient plasma are compared to dilutions of a plasma pool from normal donors, when added to deficient plasma. Levels are reduced by hereditary (Hemophilia B) or acquired factor IX deficiency, factor IX inhibitor, vitamin K deficiency, warfarin therapy, or liver disease.
Additional Test Information Normal Range: 68-132% effective 11/1/11
Sample Requirements Whole Blood: 3.2% Sodium Citrated (blue top) OR
Frozen Plasma
Requested Volume Two 5 ml 3.2 sodium citrated (blue top) tubes as whole blood OR
Frozen plasma in 4 X 1mL aliquots in plastic tubes
Minimum Volume or Pediatric volume Frozen Plasma: at least 0.5ml in 2 plastic tubes.
Sample Information Whole blood is preferred for testing but if it is not possible to send whole blood tubes TO ARRIVE HERE WITHIN 3 HOURS of collection then send frozen plasma. To prepare plasma centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes. Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze.
Shipping Information Whole Blood: Send at ambient temperature (18-25C) and must be received within the lab in less than or equal to 3 hours after collection.
Frozen Plasma: send on dry iceSend samples to:Bloodworks Northwest
Attn: Hemostasis Laboratory
921 Terry Ave
Seattle, WA 98104For additional information, please see Ordering Information
Requisition Form RFT- Hemostasis Reference Laboratory
Transaction Code 3210-14
CPT Codes 85250
Test Schedule Monday – Friday
Turn around Time (analytic time) 1 Business Day
Report/ Results Fax number required to receive report

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