Bloodworks Northwest Laboratories
Donor Testing-Quality Control Laboratory
Minimum Sample Requirement
| Test | Maximum Sample Age Stored at 2-8°C | Acceptable Sample Type | ||||
| Whole Blood Samples | Sample Aliquot Min. Volume | Serum
(From Clot Tube) Acceptable? |
EDTA Plasma Acceptable? | Comments | ||
| Donor Battery | 3 days | 1 X 6 mL Clot plus 2 X 6 mL EDTA tubes | ||||
| Recipient/ Patient Battery | 5 days | 1 X 6 mL Clot or 1 X 6 mL EDTA1 tube | 4 mL | Y | Y1 | 1If EDTA submitted, sample acceptable for 48 hours for STS testing. |
| HBsAg | 14 days | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 2 mL | Y | Y | |
| a-HBc | 14 days | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 1 mL | Y | Y | |
| a-HIV-1/-2 | 14 days | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 1 mL | Y | Y | |
| a-HTLV-I/-II | 14 days | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 1 mL | Y | Y | |
| a-HCV | 14 days | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 1 mL | Y | Y | |
| a-CMV | 7 days | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 1 mL | Y | Y | |
| STS by RPR | 5 days | 1 X 6 mL Clot or 1 X 6 mL EDTA2 tube | 0.5 mL | Y | Y2 | 2EDTA acceptable up to 48 hours.
Serum is required for confirmatory testing or when >48 hours since collection. |
| Chagas | 14 days | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 0.5 mL | Y | Y | |
| HCV-HIV-HBV NAT
(Living Donor) |
12 days3,4 | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 3 mL | N | Y | To extend shelf life, refrigerate immediately and remove plasma from cells using a sterile pipette tip.
3Plasma, Becton-Dickinson PPT, and serum clot tubes acceptable up to 72 hours at 2-25°C. To extend storage up to 12 days, samples must be centrifuged within 72 hours. 4Blood collected in serum clot tubes can be stored up to 7 days at 2-8°C but must be centrifuged within 72 hours. |
| WNV NAT
(Living Donor) |
5 days5 | 1 X 6 mL EDTA tube | NA | N | Y | 5EDTA acceptable for only 3 days if stored between 8 and 25°C |
| ZIKA NAT (Living Donor) | 5 days6 | 1 X 6 mL EDTA tube | NA | N | Y | 6EDTA or PPT acceptable for only 3 days if stored between 8 and 25°C |
| EBV VCA | 2 days | 1 X 6 mL Clot | 0.5 mL | Y | N | Serum only |
| EBV NA | 2 days | 1 X 6 mL Clot | 0.5 mL | Y | N | Serum only |
| Toxoplasma | 2 days | 1 X 6 mL Clot | 0.5 mL | Y | N | Serum only |
| HBsAg Confirmatory by Abbott | 14 days | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 3 mL | Y | Y | |
| HBsAg Confirmatory by Bio-Rad | 7 days | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 1 mL | Y | Y | |
| a-HIV-1/-2 Confirmatory | 7 days | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 2 mL | Y | Y | |
| a-HTLV-I/-II Confirmatory | 14 days | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 2 mL | Y | Y | |
| a-HCV Alternate Assay | 10 days | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 1 mL | Y | Y | |
| Cadaveric Specific Sample Requirements | ||||||
| HCV-HIV-HBV NAT | 2 days7 | 1 X 6 mL Clot tube | 3 mL | Y | N | 7Samples can be stored at 2-25°C for 48 hours. Separated serum acceptable for additional 8 days if stored at 2-8°C |
| HBsAg by Bio-Rad | 7 days | 1 X 6 mL Clot | 1 mL | Y | N | |
| a-HBc by Ortho | 10 days8 | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 8Whole Blood limited to 24 hours if stored 8-25°C | |||
| a-HIV-1/2 by Bio-Rad | 7 days | 1 X 6 mL Clot | 1 mL | Y | N | |
| a-HTLV-I/-II by Avioq | 14 days | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 1 mL | Y | Y | |
| a-HCV by Ortho | 10 days9 | 1 X 6 mL Clot or 1 X 6 mL EDTA tube | 1 mL | Y | Y | 9Whole Blood limited to 24 hours if stored 8-25°C |
Quick Links
- Request for Testing
- Ordering Information
- Sample Requirements
- Licenses & Accreditations
- Laboratory Leadership
- References
Contact us: 425-656-7907
