Questions and Answers about Coronavirus

Bloodworks Northwest Laboratories

Donor Testing-Quality Control Laboratory

Minimum Sample Requirement

Test Maximum Sample Age Stored at 2-8°C Acceptable Sample Type
Whole Blood Samples Sample Aliquot Min. Volume Serum

 (From Clot Tube) Acceptable?

EDTA Plasma Acceptable? Comments
Donor Battery 5 days 1 X 6 mL Clot plus 2 X 6 mL EDTA tubes
Recipient/ Patient Battery 5 days 1 X 6 mL Clot or 1 X 6 mL EDTA1 tube 4 mL Y Y1 1If EDTA submitted, sample acceptable for 48 hours for STS testing.
HBsAg 14 days 1 X 6 mL Clot or 1 X 6 mL EDTA tube 2 mL Y Y
a-HBc 14 days 1 X 6 mL Clot or 1 X 6 mL EDTA tube 1 mL Y Y
a-HIV-1/-2 14 days 1 X 6 mL Clot or 1 X 6 mL EDTA tube 1 mL Y Y
a-HTLV-I/-II 14 days 1 X 6 mL Clot or 1 X 6 mL EDTA tube 1 mL Y Y
a-HCV 14 days 1 X 6 mL Clot or 1 X 6 mL EDTA tube 1 mL Y Y
a-CMV 7 days 1 X 6 mL Clot or 1 X 6 mL EDTA tube 1 mL Y Y
STS by RPR 5 days 1 X 6 mL Clot or 1 X 6 mL EDTA2 tube 0.5 mL Y Y2 2EDTA acceptable up to 48 hours.

Serum is required for confirmatory testing or when >48 hours since collection.

Chagas 14 days 1 X 6 mL Clot or 1 X 6 mL EDTA tube 0.5 mL Y Y
HCV-HIV-HBV NAT

(Living Donor)

12 days3,4 1 X 6 mL Clot or 1 X 6 mL EDTA tube 3 mL N Y To extend shelf life, refrigerate immediately and remove plasma from cells using a sterile pipette tip.

 

3Plasma, Becton-Dickinson PPT, and serum clot tubes acceptable up to 72 hours at 2-25°C. To extend storage up to 12 days, samples must be centrifuged within 72 hours.

4Blood collected in serum clot tubes can be stored up to 7 days at 2-8°C but must be centrifuged within 72 hours.

WNV NAT

(Living Donor)

5 days5 1 X 6 mL  EDTA tube NA N Y 5EDTA acceptable for only 3 days if stored between 8 and 25°C
ZIKA NAT (Living Donor) 5 days6 1 X 6 mL  EDTA tube NA N Y 6EDTA or PPT acceptable for only 3 days if stored between 8 and 25°C
EBV VCA 2 days 1 X 6 mL Clot 0.5 mL Y N Serum only
EBV NA 2 days 1 X 6 mL Clot 0.5 mL Y N Serum only
Toxoplasma 2 days 1 X 6 mL Clot 0.5 mL Y N Serum only
HBsAg Confirmatory by Abbott 14 days 1 X 6 mL Clot or 1 X 6 mL EDTA tube 3 mL Y Y
HBsAg Confirmatory by  Bio-Rad 7 days 1 X 6 mL Clot or 1 X 6 mL EDTA tube 1 mL Y Y
a-HIV-1/-2 Confirmatory 7 days 1 X 6 mL Clot or 1 X 6 mL EDTA tube 2 mL Y Y
a-HTLV-I/-II Confirmatory 14 days 1 X 6 mL Clot or 1 X 6 mL EDTA tube 2 mL Y Y
a-HCV Alternate Assay 10 days 1 X 6 mL Clot or 1 X 6 mL EDTA tube 1 mL Y Y
Cadaveric Specific Sample Requirements
HCV-HIV-HBV NAT 2 days7 1 X 6 mL Clot tube 3 mL Y N 7Samples can be stored at 2-25°C for 48 hours. Separated serum acceptable for additional 8 days if stored at 2-8°C
HBsAg by Bio-Rad 7 days 1 X 6 mL Clot 1 mL Y N
a-HBc by Ortho 10 days8 1 X 6 mL Clot or 1 X 6 mL EDTA tube 8Whole Blood limited to 24 hours if stored 8-25°C
a-HIV-1/2 by Bio-Rad 7 days 1 X 6 mL Clot 1 mL Y N  
a-HTLV-I/-II by Avioq 14 days 1 X 6 mL Clot or 1 X 6 mL EDTA tube 1 mL Y Y
a-HCV by Ortho 10 days9 1 X 6 mL Clot or 1 X 6 mL EDTA tube 1 mL Y Y 9Whole Blood limited to 24 hours if stored 8-25°C

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